prism 6.0 software enzyme kinetics package Search Results


cell lines human hl 60 hl 60  (ATCC)
99
ATCC cell lines human hl 60 hl 60
Cell Lines Human Hl 60 Hl 60, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bstui enzyme  (New England Biolabs)
96
New England Biolabs bstui enzyme
Bstui Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tween 20 horseradish peroxidase activity  (Thermo Fisher)
98
Thermo Fisher tween 20 horseradish peroxidase activity
Tween 20 Horseradish Peroxidase Activity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnase i  (Thermo Fisher)
99
Thermo Fisher dnase i
Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snp cyp2b6 c 7817765 60  (Thermo Fisher)
97
Thermo Fisher snp cyp2b6 c 7817765 60
Snp Cyp2b6 C 7817765 60, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tryple  (Thermo Fisher)
99
Thermo Fisher tryple
Tryple, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trichodesmium erythraeum  (ATCC)
93
ATCC trichodesmium erythraeum
Trichodesmium Erythraeum, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse rat fgf 23 intact elisa kit  (Immutopics Inc)
86
Immutopics Inc mouse rat fgf 23 intact elisa kit
Mouse Rat Fgf 23 Intact Elisa Kit, supplied by Immutopics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pfu-polymerase  (Promega)
90
Promega pfu-polymerase
Pfu Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kit cdiff check tm- 60  (TechLab Inc)
90
TechLab Inc elisa kit cdiff check tm- 60
The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
Elisa Kit Cdiff Check Tm 60, supplied by TechLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buffer solution  (Merck KGaA)
90
Merck KGaA buffer solution
The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
Buffer Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bstap i restriction endonuclease  (New England Biolabs)
94
New England Biolabs bstap i restriction endonuclease
The gluD homologues from closely related bacterial species were expressed in the C <t>.</t> <t>difficile</t> gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and <t>ELISA</t> ( E & H ).
Bstap I Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The gluD homologues from closely related bacterial species were expressed in the C . difficile gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and ELISA ( E & H ).

Journal: PLoS ONE

Article Title: Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo

doi: 10.1371/journal.pone.0160107

Figure Lengend Snippet: The gluD homologues from closely related bacterial species were expressed in the C . difficile gluD mutant strain, and their secretion from C . difficile was analyzed. Cytosolic (cyt) and concentrated supernatants (sup) from the bacterial cultures expressing various gluD constructs were separated by SDS-PAGE, and were analyzed by Coomassie staining (A) and by zymogram (B) . C . difficile gluD constructs with deletions of their N-terminus (panels C , D, and E ) or of C-terminus (panels F , G and H ) were expressed in C . difficile gluD mutant, and their secretion from C . difficile was analyzed by zymogram ( D & G ) and ELISA ( E & H ).

Article Snippet: The presence of C . difficile GDH in the cecal contents was detected with a commercially available ELISA kit (CDiff Check TM- 60, TechLab Inc., Blacksburg, Va.) in accordance with the manufacturer's instructions.

Techniques: Mutagenesis, Expressing, Construct, SDS Page, Staining, Enzyme-linked Immunosorbent Assay